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Dr. Monisha Khanna

Dr. Monisha Khanna, is not only an excellent teacher but also a good researcher. Students respect her for dedication and knowledge of her subject Molecular biology and Biotechnology. She always works towards preset goals. She is to be found busy in her research laboratory at all times.

Title Dr. First Name Monisha Last Name Khanna Photograph
Designation Associate Professor  
Department Zoology

Address
(Campus)

Acharya Narendra Dev College
Govind Puri,
Kalkaji
New Delhi-110019.

Phone Number
(Campus)

011-26293224

Fax

011-26294540

Email

monisha_khanna@hotmail.com

Web Page

http://andcollege.du.ac.in
Education Subject Institution
B.Sc(H) Zoology S.G.T.B KHALSA COLLEGE, UNIVERSITY OF DELHI
M.Sc Zoology DEPARTMENT OF ZOOLOGY, UNIVERSITY OF DELHI
Ph.D Molecular Biology DEPARTMENT OF ZOOLOGY, UNIVERSITY OF DELHI
Research Interests/ Specialization

I have done my Ph.D. in Molecular Biology from the Department of Zoology Following is a brief summary of my Ph.D. thesis:

CONSTRUCTION OF AN IMPROVED CLONING VECTOR AND THE  DEVELOPMENT OF AN EFFICIENT TRANSFORMATION SYSTEM FOR THE RIFAMYCIN PRODUCER

Amycolatopsis mediterranei (Nocardia mediterranei)

Rifamycins are macrolide antibiotics commanding a high therapeu¬tic status in the treatment regimen of tuberculosis and leprosy. These polyketide drugs are produced as secondary metabolites primarily by the filamentous bacterium - Amycolatopsis mediterra¬nei which is closely related to the genus Streptomyces. Inspite of the ever increasing consumption of anti-TB drugs in India (from 200 MT in 1995 to 300 MT in 1999-2000), the requirement of this bulk drug was met largely by imports with indigenization having been initiated only in 1992.

Efforts of several research groups all over the world have led to the development of strains of A. mediterranei with enhanced capabilities of rifamycin production. This strain improvement has been brought about exclusively by traditional methods such as mutation - selection, genetic recombination, and tuning of fermen¬tation parameters.

However, there is a limited scope of improvement that can be attained by such empirical methods and the focus has automatically shifted to the more versatile methods of recombinant DNA technology. As a result appreciable advances have been made in the cloning and characterization of several antibiotic biosynthetic genes coding for antibiotics like actinorhodin, granaticin, frenolicin, daunorubicin, etc. which are produced by various species of Streptomyces. Rapid progress in this field has been made possible by the development and refining of a variety of plasmid cloning vectors and efficient systems of transformation. This has now opened up the possibilities of further manipulation of Strepto¬myces spp. for the production of new and hybrid antibiotics. However, species of Amycolatopsis which apart from rifamycins, produce several other antibiotics remained out of the scope of recombinant DNA techniques. This was due to the fact that the development of comparable gene cloning methods in A. mediterranei have long been hampered by the unsuitability of Streptomyces plasmids and transformation procedures based on protoplasting and PEG treatment.

A major breakthrough in this regard was the construction of a hybrid vector – pRL1 (10.4kb), which could shuttle between A. mediterranei and E. coli (Lal et al. 1991).

However, pRL1 is only a preliminary cloning vector as it has a larger size and only one marker gene (i.e. the kanamycin resistance gene). Thus there was a need to reduce its size and to introduce a second effective marker gene. The addition of such a marker also had other implications. Repeated experiments on transforma¬tion of A. mediterranei and related species revealed that these strains are intrinsically resistant to kanamycin, making it extremely difficult to select the transformants. In this con¬text, addition of a second marker gene into pRL1 or its deriva¬tives acquired a greater significance.

Thus the present investigation was undertaken with a major aim to develop a strong cloning vector and transformation system for A. mediterranei. These objectives were achieved by reducing the size of pRL1 from 10.4 to 7.4kb. The smaller plasmid generated in this way was named as pRLM10. Besides pRLM10, the size deletion of pRL1 by using the same strategy of Exonuclease III digestion also led to the generation of four other deleted plasmids. Smaller plasmids were also generated by the Bc1I partial diges¬tion strategy. In addition, the present study has also led to the successful cloning of erythromycin resistance gene (ermE) of Saccharopolyspora erythreae in pRL1. The vectors generated were named as pRLM20 and pRLM30. Restriction analysis of the recombi¬nant plasmids verified that cloning of the insert had occurred in opposite orientations. There was also a simultaneous inclusion of the multiple cloning sites (MCS) flanking erm E into pRL1.

The cloning and expression of erm E in A.mediterranei has not only made it possible to evaluate transformation efficiency accurately but also made it possible to standardize and develop a new transformation protocol for these organisms. Further more it has even facilitated the checking of the host range of pRL1. As a result, pRLM20 and pRLM30 could be transformed in the mutant strains – F1/24 and T-195, derived from the industrial strain A. mediterranei N813 obtained from Ciba-Geigy, Switzerland. This is a significant achievement as these are the strains which are likely to be manipulated for performing complementation experi¬ments and for generation of hybrid antibiotics.

The modified vectors were subsequently employed for performing electroporation experiments on A.mediterranei DSM 40773. With the routine method of electroporation, a transformation efficiency of 1 x 103 transformants/ug of DNA was obtained. Thus attempts were made to manipulate several electroporation parameters to work out the optimum condition for transformation. This led to the development of a new method which involved partial lysozyme treatment of mycelia suspended in glycerol to yield the osmo¬tially fragile spheroplasts. These could then be pulsed with high amounts of plasmid DNA to obtain a ten-fold higher transformation efficiency upto 1 x 104 transformants/ug of DNA. The field strength of 5.0 or 7.5 kV/cm and exposure time of 11-13 ms was suitable for obtaining optimum transformation efficiency.

Besides the development of efficient transformation system and cloning vectors, a bioassay technique for the rapid screening of rifamycin producers and for a subsequent analysis of their mu¬tants generated by nitrosoguanidine treatment was also developed.

Similarly, the studies involving culture morpholgy, growth pat¬tern and differences in pigmentation and antibiotic producing abilities of these organisms have provided deeper insights into the complex nature of A. mediterranei and exposed their suscepti¬bility to contamination and strain degeneration.

The results on the construction of vectors pRLM20 and pRLM30 and their ability to efficiently electrotransform several strains of the rifamycin producer A. mediterranei, is the first extensive work on the generation of efficient transformation techniques for this industrially important microorganism. The present work has thus made possible the inclusion of A. mediterranei and its allied species among the antibiotic producers, which are currently being exploited for the generation of new and hybrid antibiotics through r-DNA techniques.

    
Research interests  
I am an Associate Professor in Zoology in Acharya Narendra Dev College, a constituent College of University of Delhi. This Science College has well-equipped laboratories catering to the individual Departments. Apart from these, the College has also set up an Instrumentation Laboratory, of which I am the In-Charge. This laboratory is a Central Facility for sustaining teaching and research programs of the College. I have been the Co-Investigator for a 3-year MOEF funded Research Project. As a teacher at the Undergraduate level, it is my dream to incorporate innovative teaching strategies that provide abundant opportunities to the students for activity-based learning, project work and field studies. To achieve this aim, it becomes imperative for a teacher to keep herself abreast of the latest developments in the field of Science and Education, and to be well versed with the latest technologies. I feel that one of the best ways to achieve this target is to integrate teaching with research, especially in emerging areas, so that the students get a hands-on experience in lab work. This will not only allow them to get a better understanding of the subject, but equally important, will help the faculty to grow and to update knowledge in their field of specialization. Thus, for a person who teaches papers like Genetics, Biotechnology and Medical Zoology, where new scientific discoveries are being made routinely and the subjects are growing in leaps and bounds, I feel that research projects being run in the College, are not only desirable, but very vital for the health of the Institute and enable the faculty and students to keep pace with the rapid advances in technology. In this context, I am also providing Summer Training Workshops to selected students of the College, so that those interested in pursuing higher learning and research can be given exposure to the basic and advanced techniques in Molecular Biology.  
The MOEF sponsored research project that I had been co-ordinating was entitled “Isolation and characterization of Actinomycetes and analysis of their antibacterial potential". Following is a brief summary of the work undertaken in the project. Microbial natural products are an important source of both existing and new drugs. Among the producers of commercially important metabolites, bacteria have proven to be a prolific source with a surprisingly small group of taxa accounting for the vast majority of compounds discovered till date. Among these, Actinomycetes are the most economically and biotechnologically priceless prokaryotes. Representative genera of actinomycetes include Streptomyces, Actinomyces, Arthrobacter, Corynebacterium, Frankia, Micrococcus, Micromonospora and several others. Secondary metabolites produced by actinomycetes possess a wide range of biological activities. Although extensive work is being carried out on the isolation and characterization of actinomycetes from different environs the world over, yet very little work has been done in India on this aspect and the diverse ecological background remains unexplored. Under the project, actinomycetes from varied ecological environments have being isolated. The potential of isolates to produce novel antibiotics has been tested against known pathogenic sensitive strains. Quantitative estimation of the antimicrobial activity of the bioactive compounds has been ascertained by measuring the Minimum Inhibitory Concentrations of the powdered culture extracts.  Estimation of the tentative chemical nature of the compounds has been done by Spectrophotometer and Chromatography techniques. Selected strains have been characterized by using traditional and molecular techniques. 16S rRNA gene amplification and sequencing of the isolates has been done. Taxonomic status of the strains has been determined by constructing phylogenetic trees. Plasmid DNA isolation has been performed to check out whether the strains possess natural plasmids, that can later on be used as a cloning vectors to genetically modify the commercially relevant strains. Morphological, Biochemical and Chemotaxonomic characterization of the isolates has also been done. The strains have been submitted to M/s Nicholas Piramal Research Centre, Mumbai, who are using high through-put screens for the discovery of new bio-molecules with anti-cancer, anti-tumour, anti-fungal, anti-bacterial, anti-inflammatory and anti-diuretic potential.

A brief summary of research activities has been presented in the series of online articles on AND college by www.indiabioscience.org/node/641 online

 

Teaching Experience
  • Daulat Ram College, University of Delhi, Temporary, 9th August 1996-30th  April 1997, Courses taught- Cell Biology, Non Chordata, Chordata, Human Histology.
  • Acharya Narendra Dev College, University of Delhi, Ad Hoc, 16th July 1997-12th  March 2003.
  • Acharya Narendra Dev College, University of Delhi, Permanent, 13th March 2003 onwards, Courses taught-Genetics & Evolution, Genetics, Genomics & Biotechnology, Medical Zoology, Chordata, Biophysics & Human Genetics, Cell Biology, Immunology & Biochemistry.
Research/ Academic Projects
  • Research Experience
    Post Doctoral Research undertaken during Study Leave from College 
    Title of Study:
    To study the microbial diversity of soil bacteria and  to identify their ability to    produce  metabolites of commercial importance.
    Duration of study leave:
    16th October 2006 to 15th May 2007
    Supervisor under whom the study was undertaken:                

Professor Rup Lal
Molecular Biology Lab
Department of Zoology
University of Delhi
Delhi 11007
 

  • Research Project completed in College 
    Co-Investigator for the Research Project entitled "Isolation and characterization of actinomycetes and analysis of their antibacterial potential" funded by the Ministry of Environment and Forests (MOEF). This was a joint project between Acharya Narendra Dev College and the Department of Zoology, University of Delhi. The three year project, initiated with effect from 14th August 2006, terminated on 13.12.09 and had a total financial outlay of Rs.9.92 lacs. Click here to see the Pictures
  •  
  • Proposal entitled “Exploring useful bacteria from soil”  
  • Funding Agency: University of Delhi under scheme of innovation projects from colleges,
    Duration: May 15th 2012-May 15th 2013, Total Financial Outlay: 10lacs
      Abstract: The present project attempts to discover soil microbes possessing antimicrobial activity. Bacteria were isolated from diverse ecological habitats. Isolates were screened for antimicrobial activity against pathogenic microorganisms: Staphylococcus aureus, Bacillus cereus, Escherichia coli, Candida albicans and Fusarium oxysporum. Primary screening of strains was performed to select actinomycetes showing substantial antimicrobial activity and representing different ecological habitats. We now have a collection of several strains with antimicrobial activities and these are being subjected to further studies. Bioactive compounds have been extracted from culture broths using methanol and ethyl acetate organic solvents and concentrated to a powdered form. Stock solutions of extracts have been prepared by dissolving in suitable diluents. Activities of extracts have been quantified and compared with known antibiotics. Fractions of extracts have been separated by thin layer chromatography and then subjected to bioautography for identification of actual bioactive fractions. Bioactive compounds will further be characterized on the basis of spectrophotometric and chromatographic techniques.

  • Ongoing/Sanctioned Projects:
    • Project entitled “Extraction and activity analyses of extracellular enzymes from soil Actinomycetes”
      Funding Agency:
      University Grants Commission (UGC),
      Duration: 3 years project from May 2012 onwards, Total Financial Outlay: 9.78lacs.

      Abstract:
      Actinomycetes are an important group of soil microbes characterized by formation of branching filaments. They are not only important degraders of organic matter in the natural environment, but are also producers of antibiotics and other useful compounds of commercial interest. Actinomycetes are especially important for the production of enzymes such as chitinase, cellulase, peptidase, protease, xylanase, ligninase, amylase, sugar isomerase, pectinase, hemicellulase and keratinase. The present project will focus on production of four important enzymes: phospahatase, xylanase, chitinase and cellulase owing to their potential commercial usefulness. Phosphorus is the limiting element of crop production. It is never found in free-state but occurs naturally as calcium phosphate and as the component of many other minerals, which are not utilized directly by plants. Actinomycetes play an important role in phosphate solubilization and are used as biofertilizers for enhancing plant growth and crop production. Alkaline phosphatases are also used in molecular techniques including ligation, enzyme immunoassays and in several industrial applications like pasteurization. Microbial xylanases have commercial applications in agriculture, industry and human food production. Organisms belonging to several actinomycete genera including Streptomyces, Chainia and Thermomonospora are known to be producers of extracellular xylanases. Xylanases are used in pulp bleaching industry. Xylanases are also used as food additives to poultry,  to agricultural silage and grain feed, in wheat flour for improving dough handling and quality of baked products, for clarification of fruit juices and for degumming of plant fiber sources such as flax, hemp, jute and ramie.  Some of the most potent actinomycete producers of chitinases are Serratia marcescens, Streptomyces sp. and Trichoderma harzianum.  Chitinases are used as agents of biological control of microorganisms and of arthropod vectors.  Chitinolytic enzymes also have applications in preparation of pharmaceutically important compounds such as chitooligosaccharides with antimicrobial, antitumor and immuno-enhancing activities, for preparation of single-cell proteins, and for treatment of chitinous wastes. Cellulases have attracted much interest because of the diversity of their applications. In recent years, the interest in cellulases has increased due to many potential applications for these types of enzymes, for example, in the production of bio-energy and bio-fuel, in the textile industry and in the pulp and paper industry. Cellulases have been investigated mainly with respect to their industrial use for the bioconversion of agricultural biomass resources into useful products.
    • The proposed study will be undertaken with the following broad objectives:
      1) Isolation of actinomycetes, particularly from certain contaminated man-made habitats
      2) Primary screening or qualitative analyses of isolates for production of following enzymes-
            a) Phosphatase
            b) Xylanase      
            c) Chitinase      
            d) Cellulase
      3) Secondary screening or quantitative analyses of enzyme activity
      4) Purification of enzymes
      5) Enzyme assay of purified products.

  • Research Supervision
  • Ph.D. submission of Research scholar Renu Solanki.

Ms. Renu Solanki has become the first one to submit her Ph.D. thesis on work done at Acharya Narendra Dev College. Renu joined for Ph.D. in May 2007 and was registered as Ph.D. student on February 23, 2010 under the supervision of Dr. Monisha Khanna, Associate Professor in Zoology. She submitted her thesis entitled: ‘Isolation and characterization of actinomycetes and analysis of their antimicrobial potential’ on February 11, 2013. A brief summary of her work is as follows-

Actinomycetes were isolated from different ecological habitats using combination of physical and chemical pretreatment methods. Strains from some habitats had better antimicrobial activities as compared to others. Isolates showed noticeable activity not only against Gram positive bacteria but also against Gram negative bacteria, yeast and fungi. Primary antimicrobial analyses helped in selection of potent strains for further studies. Bioactive compounds were extracted using different solvent systems. Secondary screening and MIC determination helped in quantifying activity of various culture extracts. Some of the bioactive compounds were as effective as well known antibiotics and showed broad spectrum activities. Thin layer chromatography helped in fractionation of extracts followed by identification of actual bioactive fractions using bioautography. Structure elucidation of selected compounds revealed structures which either showed partial or complete resemblance with known antibiotics indicating the presence of novel chemical moieties in most of these extracts. These antimicrobial compounds have been identified as Actinomycin D, Mycinamicin III derivative, glycoside isomer of Mycinamicin III derivative and xantholipin derivative. Furthermore, these compounds belonged to different chemical groups including polypeptides, macrolides and xanthones. Selected strains were taxonomically characterized on the basis of microscopic, morphological, biochemical and phylogenetic studies and it was confirmed that all strains belong to genus Streptomyces. Antibiotic producing strains can further be subjected to strain improvement and combinatorial biosynthesis for production of more effective analogues or hybrid bioactive molecules.

  • Ph.D. registration of Ph.D. student Payal Das

Date of Registration: 28th March, 2013
Title of Proposed Ph.D. work: ‘Exploring useful bacteria from soil for production of secondary metabolites”.

Publications (Last Five Years)
In Indexed/Peer Reviewed Journals
2000 The importance of homologous recombination in the generation of large deletions in hybrid plasmids in Amycolatopsis mediterrane  

Plasmid. 43(1): 1-11.

 D Tuteja, M Dua, R Khanna, N Dhingra, M Khanna, H Kaur, D M Saxena, and R Lal.
 2006 Research News “Nanoorganisms: Smallest form of life got smaller" Indian Journal of Microbiology Vol 46, No. 4 R. Lal, S.Jit, M.Verma, M.Dadhwal, A.Singh, O.Prakash, P.Sharma and Monisha Khanna
2007 “Polyphasic approach of bacterial classification- An overview of recent advances" Indian Journal of Microbiology Vol 47, 98-108 O.Prakash, M.Verma, P. Sharma, M.Kumar, K. Kumari, A. Singh, H. Kumari, S. Jit, S.K. Gupta, Monisha Khanna and R. Lal
2008 Review article entitled “Bioactive compounds from marine actinomycetes" Indian Journal of Microbiology December 48: 410-431 Renu Solanki, Monisha Khanna and Rup Lal
2011

Antimicrobial activities of actinomycetes from diverse ecological habitats in Delhi and its adjoining States

Journal of Microbial World Vol 13 (2) 233-240

Renu Solanki, Rup Lal and Monisha Khanna 

2011 Bioactive compounds from soil Actinomycetes Biodiversity, Biotechnology and Man: Interdependence and future challenges” Chapter 6, pg 92-108 Monisha Khanna, Renu Solanki and Rup Lal
2011 Selective isolation of rare actinomycetes producing novel antimicrobial compounds International Journal of advanced Biotechnology and Research Vol 2 (3) 357-375 Monisha Khanna, Renu Solanki and Rup Lal
2012 Streptomyces antibioticalis, a novel species from a sanitary landfill Indian Journal of Microbiology, 52: 605-611 Monisha Khanna and Renu Solanki
2013 Metabolic profiling of actinomycetes having antimicrobial properties. International Journal of Advanced Biotechnology and Research Vol 4 (4) 444-459. Monisha Khanna, R Solanki and P Das
2014 Isolation and screening of cellulolytic actinomycetes from diverse habitats. International Journal of Advanced Biotechnology and Research Vol 5(3) 438-451. P Das, R Solanki and M Khanna
Conference Publication
Year of Publication Title Journal Co-author/s
 2006

Isolation and characterization of actinomycetes and analysis of their  antibacterial potential

Microbiology: The challenges ahead" for the 47th Annual Conference of the Association of the Microbiologists of India, held at Bhopal,December 6-8-06 Monisha Khanna and Rup Lal

 

2007

Streptomyces SL-2 and Streptomyces SL-4 spp. nov., two novel species from a sanitary landfill soil 14th International Symposium on the Biology of Actinomycetes, 26-30 August 2007 at Newcastle upon Tyne and Gateshead, UK.

Monisha Khanna and Rup Lal

2007 Soil actinomycetes and their antibacterial and antifungal potential" Microbiology:The challenges ahead" for the 48th Annual Conference of the Association of the Microbiologists of India, held at Chennai December 18-21, 2007

Renu Solanki, Monisha Khanna and Rup Lal

2008 Streptomyces chromogiensis and Streptomyces antibiotiensis spp. nov; two novel species from a sanitary landfill site. 49th Annual Microbiological  Conference held at the Department of Zoology, University of Delhi from Nov 18-20, 2008 Monisha Khanna, Renu Solanki and Rup Lal
2009 Screening of actinomycetes for novel antimicrobial compounds

50th Annual Microbiological Conference on “Third Golden Era of Microbiology" held at Pune from December 15-18 2009.

Renu Solanki, Monisha Khanna and Rup Lal
2010 Screening of soil actinomycetes for antimicrobial compounds

National Symposium on “Chemistry in Biology: The Future of Life Sciences" held at India International Centre, New Delhi from February 12-13, 2010

Renu Solanki and Monisha Khanna
2010 Streptomyces chromogiensis and Streptomyces antibiotiensis spp. nov; two novel species from a sanitary landfill site. Biodiversity, Biotechnology and Man: Interdependence and future challenges” held at Lansdowne, Uttarakhand Monisha Khanna, Renu Solanki and Rup Lal
2010. Antimicrobial activities of an actinomycete isolated from pesticide contaminated site in Lucknow, India
51st Annual Conference of AMI “International symposium on recent advances in cross disciplinary Microbiology: Avenues & Challenges” at Birla Institute of  Technology, Mesra, Ranchi Renu Solanki, Monisha Khanna and Rup Lal
2010
Two novel species of Streptomyces isolated from a sanitary landfill soil.
 National Symposium on “Biotechnology” during October 29-30, 2010 organized by Delhi Technological University, India. Monisha Khanna, Renu Solanki and Rup Lal 
2010 Bioactive compounds from soil actinomycetes
National Symposium on “Biodiversity, Biotechnology and Man: Interdependence and future challenges” held at Lansdowne, Uttarakhand
Monisha Khanna, Renu Solanki and Rup Lal
2011 Novel streptomycetes from a sanitary landfill soil International conference on “Biotechnology for Better Tomorrow” 2011, at Department of Microbiology, Dr. Babasaheb Ambedkar Marathwada University, Sub-campus, Osmanabad, Maharashtra Monisha Khanna, Renu Solanki and Rup Lal 
2011
Polyphasic characterization of actinomycetes and analysis of their antimicrobial potential, presented at the International conference on “Biotechnology for Better Tomorrow”
Department of Microbiology, Dr. Babasaheb Ambedkar Marathwada University, Sub-campus, Osmanabad, Maharashtra, India Renu Solanki, Monisha Khanna and Rup Lal 
2011
Metabolic profiling of antibiotic producing soil actinomycetes 
52nd Annual Conference of Association of Microbiologists of India (AMI) International Conference on Microbial Biotechnology for sustainable development 
Monisha Khanna, Renu Solanki and Rup Lal 
2012 Antimicrobial compounds from soil actinomycetes 
 National symposium on Microbes in health and agriculture, held at School of Life Sciences, Jawaharlal Nehru University Monisha Khanna, Renu Solanki and Rup Lal
2013
Exploring useful bacteria from soil
National conference on Redefining Science Teaching: Future of Education, held at Univesity of Delhi, South Campus, organized by Acharya Narendra Dev  College Monisha Khanna, Ravi Toteja, Vikrant Kumar, Veer , Kiran, Pooja, Munendra, Jyoti, Radha, Manav, Arpita, Megha and Khemchand
 2013 Exploring useful bacteria from soil
Antardhwani 2013, held at Rugby Stadium, University of Delhi
Monisha Khanna, Ravi Toteja, Vikrant Kumar, Veer , Kiran, Pooja, Munendra, Jyoti, Radha, Khemchand, Manav, Arpita and Megha
2013 Structure elucidation of antimicrobial compounds from actinomycetes 54th Annual conference of AMI, held at MDU,Rohtak, Haryana. Monisha Khanna, Renu Solanki and Payal Das
2013 Extracellular enzymes from soil actinomycetes 54th Annual conference of AMI, held at MDU, Rohtak, Haryana. Monisha Khanna, Renu Solanki and Payal Das
2014 “Isolation, screening and characterization of actinomycetes from diverse habitats” 55th Annual conference of AMI, held at TNAU, Coimbatore,  November 12-14, 2014

Payal Das, Renu Solanki and Monisha Khanna
Participation in Corporate Life
  • Community and Extension Services
  1. Actively participated in the Immunization Program for slum children in Najafgarh area during Pulse Polio Campaign (Oct. 1999; Jan.2000) with students of ANDC. 
  2. Participated in the Anti – AIDS Campaign of the Rotary Club (Dec. 2000).
  •  Public Outreach

The Hindi National Newspaper “Hindustan" had interviewed me (in the capacity of Head of Sericulture Department), to give information to the student community regarding Job-Oriented Degree Courses, like Sericulture, that are being offered by Delhi University Colleges. The said interview was published in its Delhi City Education Times Supplement (dated 16th June, 2005). Such type of wide publicity to a course being run exclusively by ANDC, and that too just prior to the annual Admission process ensures a good turn out of students seeking admission to this course.


  • Conferences/Seminars/Lectures/Refresher/Orientation Courses/Workshops:
  1. Won the best poster presentation award for the research paper titled “Rifamycins- Strain Improvement Program", M Khanna, H Kaur, N Srivastava, S Lal and R LaI; at the 35th Annual Conference of Association of Microbiologists of India, held at Mysore, India (Nov. 1994).
  2. Short-term training course on “Techniques in Recombinant DNA Technology" (July 1994 - Oct 1994). Sponsored by Department of Biotechnology (DBT), Government of India.
  3. Development and Higher Workshop in “Recombinant DNA Technology" for Delhi University Lecturers (Feb 1995), sponsored by Center for Professional Education (CPDHE), Delhi University. India.
  4. Short-term scientist training course on “Genetic Manipulations of Antibiotic Producing Gram Positive Bacteria" (Sept 1996 - Oct 1996), sponsored by Department of Biotechnology DBT, Government of India.
  5. Participated in the Science Exhibition “Communicating and Exploring Science: Experience of Delhi University Colleges", along with students of Daulat Ram College organized during the 84th session of Indian Science Congress (Jan 1997).
  6.  Visited the “Genetics Exhibition" organized by Department of Biotechnology at Teen Murti Bhavan, along with students of ANDC (Nov. 1998)
  7. Visited the “2nd Biotechnology India 2000 – International Exhibition and Conference" at the National Science Center, Pragati Maidan (Dec. 2000) along with students of ANDC.
  8. Certificate Course in MS Office and Internet from Informatics Computer Institute, Preet Vihar, Delhi 92 (April-May 2000).
  9. Participated in the four week Refresher Course in Environmental Sciences from Jawaharlal Nehru University (Feb.26th - March 23th 2001).
  10. Participated in the three week Refresher Course in Biotechnology from Department of Zoology, University of Delhi, conducted by the Center For Professional Development In Higher Education–CPDHE (April 12–May 2, 2001).
  11. Attended the Indo-Swiss Collaborative: Two-day Mini-Symposium on “Recent Trends In Bioremediation" held at Department of Zoology, University of Delhi, October 7- 8, 2002.
  12. Conducted the ANDC Series of Science Lectures: National symposium on “Biotechnology: Expanding Horizons" held at Auditorium, Indian National Science Academy, Bahadur Shah Zafar Marg, New Delhi 110 002, October 17, 18 2003.
  13. Participated in the two-day conference on “Microbes For The Future" held at Gargi College, University of Delhi, December 22, 24, 2003.
  14. Participated in the 4 week 64th Orientation Programme from Academic Staff College, Jamia Millia Islamia, New Delhi from 16 February-17 March 2004.
  15. Participated in Biohorizon 2004: The sixth National Symposium on Biochemical Engineering and Biotechnology from IIT Delhi, in March 2004.
  16. Participated in the 5th Annual symposium on Frontiers in Biomedical Research held at Dr. B. R. Ambedkar Center for Biomedical Research, from April 14-16, 2004.
  17. Participated in a national seminar on “Recent Trends in Chemistry" organized by Maitreyi College, University of Delhi, from September 22-24, 2004.
  18. Participated in the 3-day workshop on “Basic Techniques in Immunology" conducted by Department of Zoology, University of Delhi, August 27-29, 2005.
  19. Seminar-cum-Workshop on “Comparative endocrinology and reproductive physiology" October 4-13, 2006 held at the Department of Zoology, University of Delhi.
  20. Workshop on “Genetics, Genomics and Biotechnology" (For Zoology Teachers of Delhi University), August 10-12 2006 held at the Department of Zoology, University of Delhi.
  21. 8th Professor M.L.Bhatia Memorial Lecture “Gene therapy to combat metabolic disorders, obesity, diabetes and cardiovascular diseases" by Prof. S.P.Kalra (Department of Neurosciences, University of Florida, USA) November 14, 2006.
  22. Participated in the 2nd Asean-India Bioinformatics HRD Program supported by the Ministry of Science and Technology, and ASEAN Secretariat, and organized by Sri Venkateswara College, University of Delhi held from December 14-16, 2006 at New Delhi.
  23. Seminar on “Molecular interactions between erythroblasts and macrophages during red cell differentiation" By Manjit Hanspal, Associate Professor of Medicine, Tufts University School of Medicine, Boston, January 9, 2007, held at the Department of Zoology, University of Delhi.
  24. Attended 30th All India Cell Biology Conference and Symposium on “Molecules to compartments: Cross talks and networks" February 2-4, 2007, held at the Department of Zoology, University of Delhi.
  25. Participated in the International symposium on ciliate biology: “Biodiversity, ultrastructure, development, genome, parasitism" February 6-7, 2007 organized by SGTB Khalsa College, University of Delhi.
  26. Lecture on “The computation of navigational trajectories: Hippocampus path neurons and homing behavior in pigeons" By Prof Verner Peter Bingman, Department of Psychology, Ohio, USA, March 8, 2007, held at the Department of Zoology, University of Delhi.
  27. Lecture on “Bacterial biosensors for pollutant measurements" By Prof Van der Meer, Department of Fundamental Microbiology, University of Lausanne, Switzerland, February 14, 2007, held at the Department of Zoology, University of Delhi.
  28. “Use of Resources and www in teaching and research" organized by Delhi University on February 4, 2008, in the Lady Sri Ram College for Women, New Delhi.
  29. Actively participated in the “Delhi State Level Symposium on Forensic Science" held at S.G.T.B.Khalsa College, University of Delhi on February 14, 2008.
  30. Attended the workshop on “Biology Laboratory Course (LS-206), B.Sc. Program in Life Sciences II Year" held at Miranda House, University of Delhi, from September 24-26 2008.
  31. Attended 49th Annual Microbiological Conference “International symposium on Microbial biotechnology: Diversity, Genomics and Metagenomics" held at the Department of Zoology, University of Delhi from Nov 18th-20th 2008.
  32. Attended the “WikiED09-Workshop on Open Educational Resources" held from 9th – 13th January 2009 at Acharya Narendra Dev College.
  33. Participated in the “National Summer School on Molecular Cell Biology" held from 11th – 16th May 2009 at Sri Venkateswara College, University of Delhi.  
  34. Attended the Nineteenth Dr. C.P. Alexander Memorial Lecture on “The critical role of       taxonomy and reference collections in bioprotection and biodiversity" held on 18th December at Department of Zoology, University of Delhi
  35. Participated in the National Symposium on “Chemistry in Biology: The future of Life Sciences" held at India International Centre, New Delhi from 12th to 13th February 2010.
  36. Participated in the National Symposium on “Biodiversity, biotechnology and man: Interdependence and future challenges” held at Lansdowne, Uttarakhand from 26th to 28th March, 2010.
  37. Participated in the National Symposium on “Biotechnology” held at Delhi Technological University, India from October 29th to 30th, 2010
  38. Attended the “Basic training workshop in Drosophila genetics” held from 13th to 14th November, 2010 at Drosophila Stock Center, Department of Zoology, University of Delhi
  39. Participated in the 51st Annual Conference of AMI “International symposium on recent advances in cross disciplinary Microbiology: Avenues & Challenges” held at Birla Institute of  Technology, Mesra, Ranchi December 14-17, 2010
  40. Participated in the International conference on “Biotechnology for Better Tomorrow” 2011, held at Department of Microbiology, Dr. Babasaheb Ambedkar Marathwada University, Sub-campus, Osmanabad, Maharashtra, India February 6-9, 2011
  41. Participated in the International conference on “Recent trends in developing bioremediation strategies for hexachlorocyclohexane (HCH) and other chlorinated contaminants” held at Department of Zoology, University of Delhi February 9-11, 2011
  42. Participated in the 52nd Annual Conference of Association of Microbiologists of India (AMI) “International Conference on Microbial Biotechnology for sustainable development” at Department of Microbiology, Panjab University, Chandigarh, India November 3-6, 2011
  43. Participated in the 6th CMS Vatavaran Environment & Wildlife Film Festival and Forum, held at Convention Cemtre, Jai Singh Road (Opp Jantar Mantar), New Delhi, December 06-10, 2011
  44. Participated in the National Symposium on “Microbes in health and agriculture”, held at School of Life Sciencces, Jawahar lal Nehru University, Delhi, from March 12-13, 2012.
  45. Participated in mega multidimensional event of University of Delhi- ‘Antardhwani 2013’ from February 22-24, 013.
  46. Participated in the National Conference on ‘‘Redefining Science teaching: Future of Education’ held at University of Delhi, South campus, March 7-9, 2013.
  47. Participated in International conference on Emerging Trends of Nanotechnology in Drug Discovery, organized by Sri Venkateswara College, University of Delhi and Department of Biochemistry, UDSC India in association with CQM, University of Madeira, Portugal, held at University of Delhi, South Campus, May 26-27, 2014
  • Administrative Experience
    • Teacher-In –Charge (2004-2006) of two Departments: Zoology and Sericulture
    • Convenor of Admissions for the entire College, for the academic years 2005-2007
    • In-Charge (2004-05 and 2007 onwards) of an UGC funded Instrumentation Laboratory in the College to facilitate Higher Learning for the student community and to promote Research Activities among the faculty members.  
    • Joint Secretary (2003) of the Inter-Departmental team involved in organizing a National symposium on “Biotechnology: Expanding Horizons" held at the Indian National Science Academy, Bahadur Shah Zafar Marg, New Delhi 110 002, October 17, 18 2003
    • Member of Alumni Affairs Committee 2010-2012
    • Member of Child Care Leave Committee 2012-2014
    • Member of College Governing Body 2011-2012
    • Member of College Purchase Committee from 2004 onwards
  • Convener of Biomedical Sciences for the academic year 1998-1999 
  1. Member of the following College Committees:
  2. Time Table and Work-Load Committee (2004-06)
  3. Asset Management Committee (2004-06)
  4. Proctorial Committee (2006-08)
  5. Debating Society
  6. Environmental Club                
  7. Gardening Committee
  8. Women Students Affairs Committee “SASHAKT"
  9. Member of the Asset Management Committee for the year 2007-08
  10. Member of the College Development Committee for the years 2008-10

 

Workshops Conducted

I was the resource person and/or the Co-ordinator for the following workshops:

  1. Short-term training course on “Techniques in Recombinant DNA Technology" (July 1994 - Oct 1994). Sponsored by Department of Biotechnology (DBT), Government of India
  2. Development and Higher Workshop in “Recombinant DNA Technology" for Delhi University Lecturers (Feb 1995), sponsored by Center for Professional Education (CPDHE), Delhi University. India.
  3. Short-term scientist training course on “Genetic Manipulations of Antibiotic Producing Gram Positive Bacteria" (Sept 1996 - Oct 1996), sponsored by Department of Biotechnology DBT, Government of India.
  4. Conducted the ANDC Series of Science Lectures: National symposium on “Biotechnology: Expanding Horizons" held at Auditorium, Indian National Science Academy, Bahadur Shah Zafar Marg, New Delhi 110 002, October 17, 18 2003.
  5. I was the Co-ordinator and Resource Person for the Summer Training Workshop for B.Sc (H) Zoology Part III students on “Basic Techniques in Molecular Biology" held at A.N.D.C from June 1st to 30th 2008.
  6. Organized a four day Workshop for College Teachers for imparting technical skills in practical exercises of B.Sc Program Life Sciences Part III, from Oct 21st-24th 2008 in Acharya Narendra Dev College. 
  7. I was the Co-ordinator and Resource Person for the Summer Training Workshop for B.Sc (H) Zoology Parts II & III students on “Biology and Biotechnology of Actinomycetes" held at A.N.D.C from June 8th to 30th June 2009.
  8. Co-ordinator and Resource Person for the Summer Training Workshop for students on “Polyphasic characterization of actinomycetes and analysis of their antimicrobial potential” held at A.N.D.C from May 15th to August 10th 2010.
  9. Convenor for the two day Hands-on-Training on “Molecular Phylogenetics: Understanding Evolutionary Histories” organized by Acharya Narendra Dev College, University of Delhi, held on June 15-16, 2011. Click Here to view the Pictures of this Workshop.
  10. Co-ordinator and Resource Person for the Summer Training Workshop for students on “Isolation of antimicrobial compounds from soil actinomycetes” held at A.N.D.C from 22nd June to 19th July, 2011.Click Here to view the Pictures of this Workshop
  11. Co-ordinator and Resource Person for workshop on “Experimental Procedures of Cell and Molecular Biology” for Botany Teachers held at Zoology Department A.N.D.C on 9th September, 2011 Click Here to view the Pictures of this Workshop

Professional Societies Memberships
  1. Life Member of Journal of Microbial World 
  2. Member of Department Research Committee (DRC), Zoology Department, University of Delhi
  3. Life Member of AMI (Association of Microbiologists of India)
  4. I have been a member of the Working Committee involved in framing of Zoology E-Books for the Undergraduate Curriculum. This 45 crores National Project of setting up a National Science Digital Library (NSDL) had been launched by the National Institute of Science Communication and Information Resources (NISCAIR) CSIR. 
  5. Referee / Reviewer for the “Indian Journal of Microbiology"
  6. Involved in Curriculum Development for various Undergraduate Courses:
    Member of the Committee “Genetics, Genomics and Biotechnology" for framing syllabus for B.Sc. (Hons.) Zoology. Also, a member of the Committee “Cell and Molecular Biology" for B.Sc. (Gen.) and a Co-Convenor of the Committee “Applied Zoology" also for B.Sc. (Gen.) courses. Several such committees were formed by the Department of Zoology, Delhi University, and all these have been involved in revising and updating the syllabus of B.Sc. (Hons, Gen, and Subsid) Courses in   Zoology, that have been implemented in a phased manner (2004-2006)
    Involved in restructuring the syllabus for B.Sc. Applied Life Sciences (Sericulture) Parts II and III, implemented from the academic year (2006-08)
  7. Member of the Working Committee involved in framing of revised syllabi for the Semester courses in Zoology and B.Sc. Program in Life Sciences to be introduced at the Undergraduate level in the coming academic session. I am working on syllabus modification for the following papers:

  (i) Genetics and Genomics: Two papers
  (ii) Animal Diversity (Chordates): Two papers
  (iii) Biotechnology: One paper 
  (iv) Animal Diversity for Life Sciences: One paper
  (v) Genetics and Genomics for Life Sciences: One paper
  (vi) Applied Biology and Biotechnology: One paper